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Your Position: Home > Receptor Tyrosine Kinases > Her2 > Human HER2 Protein,Tag Free

Human Her2 / ErbB2 Protein, Tag Free

  • Synonym
    ERBB2,CD340,HER-2/neu,HER2,MLN19,NEU,NGL,TKR1
  • Source
    Human Her2, Tag Free (HE2-H521y) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # P04626-1).
    Predicted N-terminus: Thr 23
  • Molecular Characterization
    Her2(Thr 23 - Thr 652) P04626-1

    This protein carries no "tag".

    The protein has a calculated MW of 70.1 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Her2, Tag Free (Cat. No. HE2-H521y) SDS-PAGE gel

Human Her2, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human Her2, Tag FreeHuman Her2, Tag Free (Cat. No. HE2-H521y) ELISA bioactivity

Immobilized Human Her2, Tag Free (Cat. No. HE2-H521y) at 2 μg/mL (100 μL/well) can bind Trastuzumab with a linear range of 0.4-3 ng/mL (QC tested). (The Peroxidase AffiniPure Rabbit Anti-Human IgG, F(ab')2 Fragment Specific reagent is used as secondary antibody in the assay)

  • Background
    Human Epidermal growth factor Receptor 2 (HER2),also called ERBB2, HER-2,HER-2/neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer,communication at the neuromuscular junction andregulation of cell growth and differentiation.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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