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Your Position: Home > Receptor Tyrosine Kinases > Her2 > Human Her2 / ErbB2 Protein, Tag Free

Human Her2 / ErbB2 Protein, Tag Free

  • Synonym
    ERBB2,CD340,HER-2/neu,HER2,MLN19,NEU,NGL,TKR1
  • Source
    Human Her2, Tag Free (HE2-H521y) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # P04626-1).
    Predicted N-terminus: Thr 23
  • Molecular Characterization
    Online(Thr 23 - Thr 652) P04626-1

    This protein carries no "tag".

    The protein has a calculated MW of 70.1 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Her2, Tag Free (Cat. No. HE2-H521y) SDS-PAGE gel

Human Her2, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human Her2, Tag FreeHuman Her2, Tag Free (Cat. No. HE2-H521y) ELISA bioactivity

Immobilized Human Her2, Tag Free (Cat. No. HE2-H521y) at 2 μg/mL (100 μL/well) can bind Trastuzumab with a linear range of 0.4-3 ng/mL (QC tested). (The Peroxidase AffiniPure Rabbit Anti-Human IgG, F(ab')2 Fragment Specific reagent is used as secondary antibody in the assay)

Please do not use together with anti human IgG Fc secondary antibody in order to avoid high background.

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