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Your Position: Home > MABSol® Biotin Labeled Proteins > Human EpCAM /CD326 Protein, Biotinylated

Biotinylated Human EpCAM / TROP1, ultra sensitivity (primary amine labeling)

  • Synonym
    EPCAM,TACSTD1,TROP1,CD326,DIAR5,EGP2,EGP314,EGP40,ESA,GA733-2,HNPCC8,HNPCC-8,KS1/4,KSA,M4S1,MIC18,MK1
  • Source
    MABSol® Biotinylated Human EpCAM / TROP1 (EPM-H8223) is expressed from human HEK293 cells. It contains AA Gln 24 - Lys 265 (Accession # AAH14785.1). It is the biotinylated form of Human EpCAM / TROP1 Protein (Cat # EPM-H5223).
    Predicted N-terminus: Gln 24
  • Molecular Characterization
    EpCAM(Gln 24 - Lys 265) AAH14785.1

    This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 29 kDa. The protein migrates as 33-36 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 6-7 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human EpCAM (Cat. No. EPM-H8223) SDS-PAGE gel

Biotinylated Human EpCAM on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

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Price(USD) : $290.00

Inventory : In stock

Price(USD) : $1250.00

Inventory : In stock

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