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Your Position: Home > All Other Proteins > Mesothelin > Biotinylated Human MSLN /Mesothelin (aa 296-580) Protein,N-Fc Tag

Biotinylated Human Mesothelin / MSLN (296-580), Fc Tag, ultra sensitivity (primary amine labeling)

  • Synonym
    MSLN,Mesothelin,MPF
  • Source
    MABSol® Biotinylated Human Mesothelin / MSLN (296-580), Fc Tag (MSN-H826x) is expressed from human HEK293 cells. It contains AA Glu 296 - Gly 580 (Accession # AAH09272). It is the biotinylated form of Human Mesothelin / MSLN (296-580) Protein, Fc Tag (MSN-H526x).
    Predicted N-terminus: Gly
  • Molecular Characterization
    Mesothelin(Glu 296 - Gly 580) AAH09272

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 59.6 kDa. The protein migrates as 60-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 3-5 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Mesothelin (aa 296-580), Fc tag (Cat. No. MSN-H826x) SDS-PAGE gel

Biotinylated Human Mesothelin (aa 296-580), Fc tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human Mesothelin (aa 296-580), Fc tagBiotinylated Human Mesothelin (aa 296-580), Fc tag (Cat. No. MSN-H826x) ELISA bioactivity

Immobilized Anti-Human MSLN Mab at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Mesothelin, Fc Tag (Cat. No. MSN-H826x) with a linear range of 0.5-8 ng/mL (QC tested).

Evaluation of CAR expression
FACS Analysis of Anti-MSLN CAR Expression
Evaluation of CAR expression

293 cells were transfected with anti-MSLN-scFv and RFP tag. 2e5 of the cells were first stained with B. Biotinylated Human MSLN (296-580), Fc Tag (Cat. No. MSN-H826x, 3 µg/ml) and C. Biotinylated Protein Control, followed by FITC Streptavidin. A. Non-transfected 293 cells and C. Biotinylated Protein Control were used as negative control. RFP was used to evaluate CAR (anti-MSLN-scFv) expression and FITC was used to evaluate the binding activity of Biotinylated Human MSLN (296-580), Fc Tag (Cat. No. MSN-H826x).

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Price(USD) : $290.00

Price(USD) : $1250.00

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