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Biotinylated Recombinant Protein L, ultra sensitivity (primary amine labeling, long spacer)

  • Synonym
    RPL,Protein L
  • Source
    Biotinylated Recombinant Protein L, Superior Sensitivity (RPL-P814R) is expressed from E.coli cells. It is the biotinylated form of Recombinant Protein L (RPL-P3141).
  • Molecular Characterization

    This protein carries a polyhistidine tag at the N-terminus. The protein migrates as 50-60 kDa on a SDS-PAGE gel under reducing (R) condition. This protein can bind to VL-Kappa.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A specially optimized long-spacer biotin reagent (32.5 angstroms) is used in this product to minimize potential steric hindrance.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 8-10.5 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Recombinant Protein L, primary amine labeling, long spacer (Cat. No. RPL-P814R) SDS-PAGE gel

Biotinylated Recombinant Protein L, primary amine labeling, long spacer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Biotinylated Recombinant Protein L, primary amine labeling, long spacerBiotinylated Recombinant Protein L, primary amine labeling, long spacer (Cat. No. RPL-P814R) ELISA bioactivity

Immobilized Anti-Human CTLA4 MAb at 5 μg/mL (100 μL/well)can bind Biotinylated Recombinant Protein L (Cat. No. RPL-P814R) with a linear range of 0.1-1 ng/mL (QC tested).

Evaluation of CAR expression
FACS Analysis of Anti-BCMA CAR Expression
Evaluation of CAR expression

293 cells were transfected with anti-BCMA-scFv and RFP tag. 2e5 of the cells were first stained with Biotinylated Recombinant Protein L, Superior Sensitivity (Cat. No. RPL-P814R, 20 µg/ml), followed by FITC Streptavidin. FITC Streptavidin was used as negative control.

  • Background
    Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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