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Your Position: Home > All Other Proteins > CD3 epsilon > Human CD3E /CD3 Epsilon Protein, Biotinylated

Biotinylated Human CD3 epsilon, ultra sensitivity (primary amine labeling)

  • Synonym
    FLJ18683,T3E,TCRE,CD3E,CD3-epsilon
  • Source
    MABSol® Biotinylated Human CD3 epsilon (CDE-H8223) is expressed from human HEK293 cells. It contains AA Asp 23 - Asp 126 (Accession # NP_000724.1). It is the biotinylated form of Human CD3 epsilon Protein (Cat. No. CDE-H5223).
    Predicted N-terminus: Asp 23
  • Molecular Characterization
    CD3E(Asp 23 - Asp 126) NP_000724.1

    This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 13.2 kDa. The protein migrates as 15-16 kDa on a SDS-PAGE gel under reducing (R) condition.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-2 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human CD3 epsilon (Cat. No. CDE-H8223) SDS-PAGE gel

Biotinylated Human CD3 epsilon on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Biotinylated Human CD3 epsilonBiotinylated Human CD3 epsilon (Cat. No. CDE-H8223) ELISA bioactivity

Immobilized Anti Human CD3 MAb, mouse IgG1 (Clone # SP34-2) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD3E, His Tag (Cat. No. CDE-H8223) with a linear range of 0.05-2 ng/mL (QC tested).

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Price(USD) : $350.00

Price(USD) : $1380.00

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