>Glycoprotein / GP (virus)
>Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) Envelope Glycoprotein (GP), His Tag
Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) GP, His Tag, containing GP1 and GP2, is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 67.0 kDa. The reducing (R) protein migrates as 21-23 kDa (GP2-delta) and 110-120 kDa (GP1) in SDS-PAGE.
>85% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) GP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.
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