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Your Position: Home > Immune Checkpoint Proteins > OX40 Ligand > OXL-H526x

Human OX40 Ligand / TNFSF4 Protein, Fc Tag

  • Synonym
    OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1,CD134 ligand,CD134L
  • Source
    Human OX40 Ligand, Fc Tag (OXL-H526x) is expressed from human 293 cells (HEK293). It contains AA Gln 51 - Leu 183 (Accession # NP_003317).
  • Molecular Characterization
    Online(Gln 51 - Leu 183) NP_003317

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 42.4 kDa. The protein migrates as 53-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human OX40 Ligand, Fc Tag (Cat. No. OXL-H526x) SDS-PAGE gel

Human OX40 Ligand, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human OX40 Ligand, Fc TagHuman OX40 Ligand, Fc Tag (Cat. No. OXL-H526x) ELISA bioactivity

Immobilized Human OX40, His Tag (Cat. No. OX0-H5224) at 0.5 μg/mL (100 μL/well) can bind Human OX40 Ligand, Fc Tag (Cat. No. OXL-H526x) with a linear range of 2-63 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human OX40 Ligand, Fc Tag (Cat. No. OXL-H526x) on Protein A Biosensor, can bind Human OX40, His Tag (Cat. No. OX0-H5224) with an affinity constant of 0.96 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

FACS assay shows that recombinant Human OX40 Ligand, Fc Tag (Cat. No. OXL-H526x) can bind to 293T cell overexpressing human OX40. The concentration of OX40 Ligand used is 0.01 μg/ml (Routinely tested).

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Order Details

Price(USD) : $290.00

Price(USD) : $1980.00

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DMF (Drug Master File)

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