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Your Position: Home > All Other Proteins > CD3E & CD3D > Biotinylated Human CD3E & CD3D Heterodimer Protein Fc Chimera

Biotinylated Human CD3E & CD3D, ultra sensitivity (primary amine labeling)

  • Synonym
    CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta / epsilon
  • Source
    MABSol® Biotinylated Human CD3 epsilon & CD3 delta (CDD-H82W0) is expressed from human HEK293 cells. It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # NP_000724.1 (CD3E) & AAH39035 (CD3D)). It is the biotinylated form of Human CD3 epsilon & CD3 delta Protein (Cat # CDD-H52W0).
    Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)
  • Molecular Characterization
    CD3E & CD3D(Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D)) NP_000724.1 (CD3E) & AAH39035 (CD3D)

    The subunit CD3E carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The subunit CD3D carries a human IgG1 Fc fragment at the C-terminus, followed by a flag tag. The protein has a calculated MW of 39.2 kDa (CD3E), 37.5 kDa (CD3D) . The protein migrates as 40-55 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 2.5-4.5 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD3E & CD3D (Cat. No. CDD-H82W0) SDS-PAGE gel

Biotinylated Human CD3E & CD3D on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Biotinylated Human CD3E & CD3DBiotinylated Human CD3E & CD3D (Cat. No. CDD-H82W0) ELISA bioactivity

Immobilized Biotinylated Human CD3E & CD3D (Cat. No. CDD-H82W0) can bind OKT3 with a linear range of 2-39 ng/mL. The biotinylated CD3E & CD3D heterodimers are coated at 1 ug/mL (100 μL/well) on a streptavidin coated plates (QC tested).

Biotinylated Human CD3E & CD3DBiotinylated Human CD3E & CD3D (Cat. No. CDD-H82W0) ELISA bioactivity

Immobilized SP34-2 at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD3E & CD3D, His Tag, Fc Tag, Flag Tag (Cat. No. CDD-H82W0) with a linear range of 1-20 ng/mL (Routinely tested).

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Price(USD) : $1250.00

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