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Your Position: Home > Fc Receptor Proteins > FcRn (FCGRT & B2M) > FCM-M52W2

Mouse FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (BLI verified)

  • Synonym
    FcRn,FCGRT & B2M
  • Source
    Mouse FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (BLI verified) (FCM-M52W2) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q61559-1 (FCGRT) & P01887-1 (B2M)).
    Predicted N-terminus: Ser 22 (FCGRT) & Ile 21 (B2M)
  • Molecular Characterization
    Online(Ser 22 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M)) Q61559-1 (FCGRT) & P01887-1 (B2M)

    Mouse FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (BLI verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 32.9 kDa (FCGRT) and 14.7 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Twin-Strep tag at the C-terminus. The reducing (R) protein migrates as 42-48 kDa (FCGRT) and 15-17 kDa (B2M) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (BLI verified) (Cat. No. FCM-M52W2) SDS-PAGE gel

Mouse FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Mouse FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (BLI verified)Mouse FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (BLI verified) (Cat. No. FCM-M52W2) ELISA bioactivity

Immobilized Ipilumimab at 5 μg/mL (100 μL/well) can bind Mouse FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (BLI verified) (Cat. No. FCM-M52W2) with a linear range of 0.625-5 μg/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Mouse FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (BLI verified) (Cat. No. FCM-M52W2) on NTA Biosensor, can bind Herceptin with an affinity constant of 0.858 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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