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Your Position: Home > Immune Checkpoint Proteins > B7-H4 > Biotinylated Human B7-H4 /B7S1 /VTCN1 Protein

Biotinylated Human B7-H4, ultra sensitivity (primary amine labeling)

  • Synonym
    B7-H4,VTCN1,B7S1,B7h.5
  • Source
    MABSol® Biotinylated Human B7-H4 (B74-H8222) is expressed from human HEK293 cells. It contains AA Phe 29 - Ala 258 (Accession # NP_078902). It is the biotinylated form of Human B7-H4 protein, His Tag (Cat. No. B74-H5222).
    Predicted N-terminus: Phe 29
  • Molecular Characterization
    Online(Phe 29 - Ala 258) NP_078902

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 26.1 kDa. The protein migrates as 43-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 3-5 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human B7-H4, His Tag (Cat. No. B74-H8222) SDS-PAGE gel

Biotinylated Human B7-H4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human B7-H4, His TagBiotinylated Human B7-H4, His Tag (Cat. No. B74-H8222) ELISA bioactivity

Immobilized Anti-Human B7-H4 MAb at 2 μg/mL (100 μL/well) can bind Biotinylated Human B7-H4, His Tag (Cat. No. B74-H8222) with a linear range of 1-8 ng/mL (QC tested).

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Price(USD) : $290.00

Price(USD) : $1250.00

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