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Your Position: Home > Fc Receptor Proteins > FcRn (FCGRT & B2M) > Biotinylated Human FcRn / FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR & BLI verified)

Biotinylated Human FcRn / FCGRT&B2M Heterodimer Protein, Avitag™,His Tag&Strep II Tag (SPR & BLI verified)

  • Synonym
    FcRn,FCGRT & B2M
  • Source
    Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR & BLI verified) (FCM-H82W4) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P55899-1 (FCGRT) & P61769-1 (B2M)).
    Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)
  • Molecular Characterization
    Online(Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M)) P55899-1 (FCGRT) & P61769-1 (B2M)

    Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR & BLI verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 34.1 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 38 kDa (FCGRT) and 14 kDa (B2M) respectively due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR & BLI verified) (Cat. No. FCM-H82W4) SDS-PAGE gel

Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR & BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR & BLI verified) (Cat. No. FCM-H82W4) captured on Biotin CAP- Series S Sensor Chip can bind Herceptin with an affinity constant of 0.369 μM as determined in a SPR assay (Biacore T200) (QC tested).

Human_FcRn_Heterodimer_Protein_SPR

Immobilized Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR & BLI verified) (Cat. No. FCM-H82W4 ) on SA Chip can bind Human HSA,His Tag, low endotoxin (Cat. No. HSA-H522a) with an affinity constant of 1.46 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Human_FcRn_Heterodimer_Protein_SPR

Immobilized Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR & BLI verified) (Cat. No. FCM-H82W4 ) on SA Chip can bind HSA, His Tag (Cat. No. HSA-H5220) with an affinity constant of 1.78 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR & BLI verified) (Cat. No. FCM-H82W4) on SA Biosensor, can bind Herceptin with an affinity constant of 0.22 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Price(USD) : $350.00

Price(USD) : $1380.00

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