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Your Position: Home > Immune Checkpoint Proteins > B7-2 > Mouse CD86 /B7-2 Protein,C-Fc Tag

Mouse B7-2 / CD86 Protein, Fc Tag

  • Synonym
    CD86,B7-2,B70,CD28LG2,LAB72,MGC34413
  • Source
    Mouse B7-2, Fc Tag (CD6-M5251) is expressed from human 293 cells (HEK293). It contains AA Val 24 - Glu 245 (Accession # NP_062261.3).
    Predicted N-terminus: Val 24
  • Molecular Characterization
    B7-2(Val 24 - Glu 245) NP_062261.3

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 52 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse B7-2, Fc Tag (Cat. No. CD6-M5251) SDS-PAGE gel

Mouse B7-2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Mouse B7-2, Fc TagMouse B7-2, Fc Tag (Cat. No. CD6-M5251) ELISA bioactivity

Immobilized Mouse B7-2, Fc Tag (Cat. No. CD6-M5251) at 5 μg/mL (100 μL/well) can bind Mouse CTLA-4, His Tag (Cat. No. CT4-M52H5) with a linear range of 0.4-13 ng/mL (QC tested).

  • Background
    Cluster of Differentiation 86 (CD86) is also known as B-lymphocyte activation antigen B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily, and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B72 is expressed at low levels on monocytes and can be upregulated through interferon γ. CD86 is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD86 works in tandem with CD80 to prime T cells. Recent study has revealed that B7-2 promotes the generation of a mature APC repertoire and promotes APC function and survival. Furthermore, the B7 proteins are also involved in innate immune responses by activating NF-κB-signaling pathway in macrophages. CD86 thus is regarded as a promising candidate for immune therapy. CD86+ macrophages in Hodgkin lymphoma patients are an independent marker for potential nonresponse to firstline-therapy.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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