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Your Position: Home > Cytokines & Growth Factors > Human-bFGF-FGF-basic-Protein

Human FGF basic Protein, Tag Free

  • Synonym
    FGF2,BFGF,FGFB,FGF basic,HBGF-2
  • Source
    Human FGF basic, Tag Free (BFF-H4117) is expressed from E.coli cells. It contains AA Pro 143 - Ser 288 (Accession # NP_001997).
    Predicted N-terminus: Met
  • Molecular Characterization
    FGF basic(Pro 143 - Ser 288) NP_001997

    This protein carries no "tag".

    The protein has a calculated MW of 16.5 kDa. The protein migrates as 17 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >98% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human FGF basic, Tag Free (Cat. No. BFF-H4117) SDS-PAGE gel

Human FGF basic, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Bioactivity
Human FGF basic, Tag FreeHuman FGF basic, Tag Free (Cat. No. BFF-H4117) ELISA bioactivity

Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 2μg/mL (100 μL/well) can bind Human Glypican 3, Fc Tag (Cat. No. GP3-H5258) with a linear range of 0.48-7.8 ng/mL (QC tested).

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