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Your Position: Home > Protein > Fc gamma RIIIA / CD16a > CDA-H82E8

Biotinylated Human Fc gamma RIIIA / CD16a (F176) Protein, Avitag™,His Tag (SPR & BLI & MALS verified)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.

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  • Synonym
    FCGR3A,CD16A,FCG3,FCGR3,IGFR3
  • Source
    Biotinylated Human CD16a (F176), Avitag,His Tag (CDA-H82E8) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Gln 208 (Accession # P08637-1).
    Predicted N-terminus: Gly 17
  • Molecular Characterization
    Online(Gly 17 - Gln 208) P08637-1

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 25.5 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD16a (F176), Avitag,His Tag (Cat. No. CDA-H82E8) SDS-PAGE gel

Biotinylated Human CD16a (F176), Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human CD16a (F176), Avitag,His Tag (Cat. No. ) MALS images

The purity of Biotinylated Human CD16a (F176), Avitag,His Tag (Cat. No. CDA-H82E8) is more than 90% and the molecular weight of this protein is around 35-54 kDa verified by SEC-MALS.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Biotinylated Human CD16a (F176), Avitag,His Tag (Cat. No. CDA-H82E8) captured on Biotin CAP- Series S Sensor Chip can bind Rituximab with an affinity constant of 1.72 μM as determined in a SPR assay (Biacore T200) (QC tested).

Human_FcRn_Heterodimer_Protein_SPR

Immobilized Biotinylated Human CD16a (F176), Avitag,His Tag (Cat. No. CDA-H82E8) on SA Chip can bind Rituximab with an affinity constant of 1.33 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human CD16a (F176), Avitag,His Tag (Cat. No. CDA-H82E8) on SA Biosensor, can bind Rituximab with an affinity constant of 1.29 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody­dependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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