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Your Position: Home > Cytokines > IL-17A > ILA-H5118

Human IL-17A / CTLA-8 Protein, Tag Free (MALS verified)

  • Synonym
  • Source
    Human IL17A, Tag Free (ILA-H5118) is expressed from E.coli cells. It contains AA Gly 24 - Ala 155 (Accession # Q16552).
    Predicted N-terminus: Met
  • Molecular Characterization
    Online(Gly 24 - Ala 155) Q16552

    This protein carries no "tag".

    The protein has a calculated MW of 15.3 kDa. the protein migrates as 18 kDa under reducing (R) condition, and 28 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Human IL17A, Tag Free (Cat. No. ILA-H5118) SDS-PAGE gel

Human IL17A, Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Human IL17A, Tag Free (Cat. No. ) MALS images

The purity of Human IL17A, Tag Free (Cat. No. ILA-H5118) was more than 95% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.

Human IL17A, Tag FreeHuman IL17A, Tag Free (Cat. No. ILA-H5118) ELISA bioactivity

Immobilized Human IL-17A, Tag Free (Cat. No. ILA-H5118) at 0.5 μg/mL (100 μL/well) can bind Human IL-17 RA, His Tag (Cat. No. ILA-H5222) with a linear range of 8-125 ng/mL (QC tested).

Human IL17A, Tag FreeHuman IL17A, Tag Free (Cat. No. ILA-H5118) ELISA bioactivity

Immobilized Human IL-17A, Tag Free (Cat. No. ILA-H5118) at 2 μg/mL (100 μL/well) can bind Anti-IL17A MAb, Human IgG1 with a linear range of 0.08-1 ng/mL (Routinely tested).


Loaded Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) on Protein A Biosensor, can bind Human IL17A, Tag Free (Cat. No. ILA-H5118) with an affinity constant of 0.21 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Human IL17/ IL17A Protein, Free Tag(Cat. No. ILA-H5118) stimulates secretion of IL-6 by NIH-3T3 cells. The ED50 for this effect is 1.444-6.941 ng/ml.

  • Background
    Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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