This protein carries no "tag".
The protein has a calculated MW of 15.3 kDa. the protein migrates as 18 kDa under reducing (R) condition, and 28 kDa under non-reducing (NR) condition (SDS-PAGE).
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Human IL17A, Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human IL-17A, Tag Free (Cat. No. ILA-H5118) at 2 μg/mL (100 μL/well) can bind Anti-IL17A MAb, Human IgG1 with a linear range of 0.08-1 ng/mL (Routinely tested).
Human IL17/ IL17A Protein, Free Tag(Cat. No. ILA-H5118) stimulates secretion of IL-6 by NIH-3T3 cells. The ED50 for this effect is 1.444-6.941 ng/ml.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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