This protein carries no "tag".
The protein has a calculated MW of 14 kDa (monomer). As a result of glycosylation, the protein migrates as 19 kDa and 20kDa under reducing (R) condition, and 40-45 kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
ActiveMax® Human VEGF121, Tag Free on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Authors: Stafford RL, et al.
Journal: Protein Eng Des Sel 2014
Application: SPR(Biacore T200)
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