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Your Position: Home > Immune Checkpoint Proteins > Human ICOS / CD278 Protein, Fc Tag

Human ICOS / CD278 Protein, Fc Tag

  • Synonym
    ICOS,CD278,AILIM
  • Source
    Human ICOS, Fc Tag (ICS-H5250) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Phe 141 (Accession # NP_036224).
    Predicted N-terminus: Glu 21
  • Molecular Characterization
    ICOS(Glu 21 - Phe 141) NP_036224

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 40.4 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    50% human ICOS (Fc Tag) and 50% human IgG1 Fc.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ICOS, Fc Tag (Cat. No. ICS-H5250) SDS-PAGE gel

Human ICOS, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is 50% of human ICOS (Fc Tag) and 50% of human IgG1 Fc.

Bioactivity
Human ICOS, Fc TagHuman ICOS, Fc Tag (Cat. No. ICS-H5250) ELISA bioactivity

Immobilized Human B7-H2, His Tag (Cat. No. B72-H5221) at 1 μg/mL (100 µl/well),can bind Human ICOS, Fc Tag (Cat. No. ICS-H5250) with a linear of 0.07-2.3 ng/mL (QC tested).

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Price(USD) : $280.00

Inventory : In stock

Price(USD) : $1700.00

Inventory : In stock

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