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Your Position: Home > All Other Proteins > CD117 > Human CD117 / c-kit Protein, Fc Tag

Human CD117 / c-kit Protein, Fc Tag

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    CD117,SCFR,c-Kit,KIT
  • Source
    Human CD117, Fc Tag (CD7-H5255) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Thr 516 (Accession # P10721-2).
    Predicted N-terminus: Gln 26
    Request for sequence
  • Molecular Characterization
    Online(Gln 26 - Thr 516) P10721-2

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 81.9 kDa. The protein migrates as 100-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD117, Fc Tag (Cat. No. CD7-H5255) SDS-PAGE gel

Human CD117, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human CD117, Fc TagHuman CD117, Fc Tag (Cat. No. CD7-H5255) ELISA bioactivity

Immobilized Human CD117, Fc Tag (Cat. No. CD7-H5255) at 2 μg/mL (100 μl/well) can bind Mouse SCF, His Tag (Cat. No. SCF-M5228) with a linear range of 0.05-1.2 ng/mL (Routinely tested).

Protocol
Human CD117, Fc TagHuman CD117, Fc Tag (Cat. No. CD7-H5255) ELISA bioactivity

Immobilized Human SCF, Tag Free (Cat. No. SCF-H4212) at 2 μg/mL (100 μL/well) can bind Human CD117, Fc Tag (Cat. No. CD7-H5255) with a linear range of 0.5-4 ng/mL (QC tested).

Protocol
  • Background
    Mast/stem cell growth factor receptor Kit (c-Kit), a member of the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily, is also known as piebald trait protein (PBT), p145 c-kit, tyrosine-protein kinase Kit and CD117,which contains five Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. CD117 acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, CD117 can activate several signaling pathways. Furthermore, Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B.
  • References

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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