The minimum detectable concentration is 100 ng/mL. Below is our recommendation on sample preparation.
|Cell Culture Supernatant||Purified Antibody||Ascites|
|1:100 dilution in PBS (optional)||0.5-2 µg/mL in PBS||1:10,000 in PBS *|
* Normally, ascites fluid contains multiple Igs. The kit can only be used to determine the relative distribution of the main isotypes.
1.Place the cassettes on a flat surface.
2.Add 100 µL sample directly to the well and wait for 5 minutes.
3.A red line should appear on the membrane with specific isotypes indicated at the same position. A red line should also appear at the “C” position.
NOTE1:Prepare samples before removing the strips from the sealed pouch.
NOTE2:Do not read after 20 minutes.
NOTE3:The color of tissue culture medium may cause high background. To address this issue, additional 40-60 µL PBS can be added into the well to clear the background.
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