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Your Position: Home > Protein > B7-1 > CD0-C5252

Cynomolgus / Rhesus macaque B7-1 / CD80 Protein, Fc Tag (MALS verified)

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  • Synonym
    CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7
  • Source
    Cynomolgus / Rhesus macaque B7-1, Fc Tag (CD0-C5252) is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # G7NXN7). In the region Val 35 - Asn 242, the AA sequence of Cynomolgus and Rhesus macaque B7-1 are homologus.
    Predicted N-terminus: Val 35
  • Molecular Characterization
    Online(Val 35 - Asn 242) G7NXN7

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 50.5 kDa. The protein migrates as 60-90 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus / Rhesus macaque B7-1, Fc Tag (Cat. No. CD0-C5252) SDS-PAGE gel

Cynomolgus / Rhesus macaque B7-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Cynomolgus / Rhesus macaque B7-1, Fc Tag (Cat. No. ) MALS images

The purity of Cynomolgus / Rhesus macaque B7-1, Fc Tag (Cat. No. CD0-C5252) is more than 90% and the molecular weight of this protein is around 115-145 kDa verified by SEC-MALS.

Bioactivity-ELISA
Cynomolgus / Rhesus macaque B7-1, Fc TagCynomolgus / Rhesus macaque B7-1, Fc Tag (Cat. No. CD0-C5252) ELISA bioactivity

Immobilized Cynomolgus / Rhesus macaque CTLA-4, His Tag (Cat. No. CT4-C5227) at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque B7-1, Fc Tag (Cat. No. CD0-C5252) with a linear range of 1-8 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Cynomolgus / Rhesus macaque B7-1, Fc Tag (Cat. No. CD0-C5252) on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) with an affinity constant of 4.5 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    B7-1 and B7-2, together with their receptors CD28 and CTLA­4, constitute one of the dominant co-stimulatory pathways that regulate T­ and B­cell responses. Although both CTLA­4 and CD28 can bind to the same ligands, CTLA­4 binds to B7­1 and B7­2 with a 20 ­ 100 fold higher affinity than CD28 and is involved in the down­regulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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