Human PCSK9 (R194A), His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 71.8 kDa. The predicted N-terminus is Gln 31. DTT-reduced Protein migrates as 20 kDa and 65 kDa in SDS-PAGE due to glycosylation and proteolytic digestion.The R194A mutation results in lower affinity of PCSK9 for LDLR.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
Human PCSK9 (R194A), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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