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Your Position: Home > Bio-Markers & CD Antigens > Human PCSK9 (R194A) Protein

Human PCSK9 (R194A) Protein

  • Synonym
    PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
  • Source
    Human PCSK9 (R194A), His Tag (PCA-H5226) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # AAI166619.1).
    Predicted N-terminus: Gln 31
  • Molecular Characterization
    PCSK9(Gln 31 - Gln 692) AAI166619.1

    Human PCSK9 (R194A), His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 71.8 kDa. The predicted N-terminus is Gln 31. DTT-reduced Protein migrates as 20 kDa and 65 kDa in SDS-PAGE due to glycosylation and proteolytic digestion.The R194A mutation results in lower affinity of PCSK9 for LDLR.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human PCSK9 (R194A), His Tag (Cat. No. PCA-H5226) SDS-PAGE gel

Human PCSK9 (R194A), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human PCSK9 (R194A), His TagHuman PCSK9 (R194A), His Tag (Cat. No. PCA-H5226) ELISA bioactivity

Immobilized Human LDL R, His Tag (Cat. No. LDR-H5224) at 10 μg/mL (100 μl/well) can bind Human PCSK9 (R194A), His Tag (Cat. No. PCA-H5226 ) with a linear range of 0.1-2 μg/mL (QC tested).

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Price(USD) : $260.00

Inventory : In stock

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