>Fc Receptor Proteins
>Fc gamma RIIA / CD32a
>Biotinylated Human Fc gamma RIIA / CD32a (R167) Protein, Avitag,His Tag (SPR & BLI verified)
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 24.0 kDa. The protein migrates as 32-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Biotinylated Human CD32a (R167), Avitag,His Tag (SPR & BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Biotinylated Human CD32a (R167), Avitag,His Tag (SPR & BLI verified) (Cat. No. CDA-H82E7) captured on Biotin CAP- Series S Sensor Chip can bind Rituximab with an affinity constant of 2.15 μM as determined in a SPR assay (Biacore T200) (QC tested).
Loaded Biotinylated Human CD32a (R167), Avitag,His Tag (SPR & BLI verified) (Cat. No. CDA-H82E7) on SA Biosensor, can bind Rituximab with an affinity constant of 0.9 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $350.00
Price(USD) : $1380.00
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