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Your Position: Home > Immune Checkpoint Proteins > 4‑1BB Ligand > Human 4-1BB Ligand / TNFSF9 Protein, Fc Tag

Human 4-1BB Ligand / TNFSF9 Protein, Fc Tag

  • Synonym
    4‑1BB Ligand,TNFSF9,CD137L
  • Source
    Human 4-1BB Ligand, Fc Tag (41L-H5257) is expressed from human 293 cells (HEK293). It contains AA Ala 50 - Glu 254 (Accession # NP_003802).
    Predicted N-terminus: Ala 50
  • Molecular Characterization
    4‑1BB Ligand(Ala 50 - Glu 254) NP_003802

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 47.4 kDa. The protein migrates as 52 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human 4-1BB Ligand, Fc Tag (Cat. No. 41L-H5257) SDS-PAGE gel

Human 4-1BB Ligand, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human 4-1BB Ligand, Fc TagHuman 4-1BB Ligand, Fc Tag (Cat. No. 41L-H5257) ELISA bioactivity

Immobilized Human 4-1BB, His Tag (Cat. No. 41B-H5227) at 100 ng/mL (100 µl/well),can bind Human 4-1BB Ligand, Fc Tag (Cat. No. 41L-H5257) with a linear of 0.1-2.5 ng/mL (Routinely tested).

Human 4-1BB Ligand, Fc TagHuman 4-1BB Ligand, Fc Tag (Cat. No. 41L-H5257) ELISA bioactivity

Immobilized Human 4‑1BB Ligand, Fc Tag (Cat. No. 41L-H5257) at 2μg/mL (100 μL/well) can bind Biotinylated Human 4-1BB, Fc Tag (Cat. No. 41B-H82F7) with a linear range of 1-7.8 ng/mL (QC tested).

  • Background
    Tumor necrosis factor ligand superfamily member 9 (4-1BBL) is also known as 4-1BB ligand, CD137L or TNFSF9, which is a cytokine that binds to TNFRSF9. 4-1BBL is the high affinity ligand of 4-1BB. 4-1BBL induces the proliferation of activated peripheral blood T-cells. Also, 4-1BBL may have a role in activation-induced cell death (AICD). Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2400.00

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