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Your Position: Home > Immune Checkpoint Proteins > PD-1 > Biotinylated Mouse PD-1 / PDCD1 Protein, Fc Tag, Enzymatically Labeled

Biotinylated Mouse PD-1 / PDCD1, Fc Tag, Avi Tag (Avitag™)

  • Synonym
    PDCD1,PD1,CD279,SLEB2
  • Source
    MABSol® Biotinylated Mouse PD-1, Fc Tag (PD1-M82F4) is expressed from human HEK293 cells. It contains AA Leu 25 - Gln 167 (Accession # NP_032824).
    Predicted N-terminus: Leu 25
  • Molecular Characterization
    Online(Leu 25 - Gln 167) NP_032824

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 44.6 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >92% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Mouse PD-1, Fc Tag (Cat. No. PD1-M82F4) SDS-PAGE gel

Biotinylated Mouse PD-1, Fc Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity-ELISA
Biotinylated Mouse PD-1, Fc TagBiotinylated Mouse PD-1, Fc Tag (Cat. No. PD1-M82F4) ELISA bioactivity

Immobilized Mouse PD-L1 / B7-H1 Protein, Fc Tag (Cat. No. PD1-M5251)at 10 μg/mL (100 μl/well) can bind Biotinylated Mouse PD-1, Fc tag (Cat. No. PD1-M82F4 ) with a linear range of 0.15-2.5 μg/mL (QC tested).

Biotinylated Mouse PD-1, Fc TagBiotinylated Mouse PD-1, Fc Tag (Cat. No. PD1-M82F4) ELISA bioactivity

Immobilized Mouse PD-L2, His Tag (Cat. No. PD2-M52E3) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse PD-1, Fc Tag (Cat. No. PD1-M82F4) with a linear range of 0.156-2.5 μg/mL (Routinely tested).

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