>Immune Checkpoint Proteins
>Biotinylated Mouse PD-1 / PDCD1 Protein, Fc Tag, Enzymatically Labeled
This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).
The protein has a calculated MW of 44.6 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>92% as determined by reduced SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Mouse PD-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
Immobilized Mouse PD-L1 / B7-H1 Protein, Fc Tag (Cat. No. PD1-M5251）at 10 μg/mL (100 μl/well) can bind Biotinylated Mouse PD-1, Fc tag (Cat. No. PD1-M82F4 ) with a linear range of 0.15-2.5 μg/mL (QC tested).
Authors: Z Lin, et al
Journal: Molecular Therapy: Oncolytics 2019
Application: PD-L1 PD-1 Blockade assay （ELISA)
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Price(USD) : $350.00
Price(USD) : $1380.00
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