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Your Position: Home > Viral Proteins > Vaccinia Virus B18R Protein

Vaccinia Virus B18R Protein

  • Synonym
    B18R
  • Source
    Vaccinia Virus B18R Protein, His 20 - Glu 351 (Accession # P25213), was produced in human 293 cells (HEK293) at ACROBiosystems.
    Predicted N-terminus: His 20
  • Molecular Characterization
    B18R(His 20 - Glu 351) P25213

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 40.3 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Vaccinia Virus  B18R, His Tag (Cat. No. B1R-H52H6) SDS-PAGE gel

Vaccinia Virus B18R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Vaccinia Virus  B18R, His TagVaccinia Virus  B18R, His Tag (Cat. No. B1R-H52H6) ELISA bioactivity

Immobilized human IFN-α1a at 2μg/mL (100 µL/well),can bind Vaccinia Virus B18R, His Tag (Cat. No. B1R-H52H6) with a linear range of 0.8-10 ng/mL (QC tested).

  • Background
    B18R is a type I interferon (IFN)-binding protein and it is encoded by the B18R open reading frame in the WR (Western Reserve) strain of vaccinia virus, which contains two Ig-like C2-type domains and one Ig-like V-type domain. B18R exists in a soluble and a membrane-bound form. As a type I IFN receptor, B18R counteracts the antiviral effects of host IFN-alpha/beta. Also, B18R acts as a soluble IFN-alpha receptor and thus inhibits the interaction between host IFN-alpha and its receptor.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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