0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Fc Receptor Proteins > FcRn (FCGRT & B2M) > FCM-M82W6

Biotinylated Mouse FcRn / FCGRT&B2M Heterodimer Protein, Avitag™,His Tag&Twin-Strep Tag (BLI verified)

  • Synonym
    FcRn,FCGRT & B2M
  • Source
    MABSol® Biotinylated Mouse FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (BLI verified) (FCM-M82W6) is expressed from human HEK293 cells. It contains AA Ser 22 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q61559-1 (FCGRT) & P01887-1 (B2M)).
    Predicted N-terminus: Ser 22 (FCGRT) & Ile 21 (B2M)
  • Molecular Characterization
    Online(Ser 22 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M)) Q61559-1 (FCGRT) & P01887-1 (B2M)

    Biotinylated Mouse FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (BLI verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 34.7 kDa (FCGRT) and 14.7 kDa (B2M). Subunit FCGRT is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag and subunit Beta-2 microglobulin (B2M) is fused with Twin-Strep tag at the C-terminus. The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 15 kDa (B2M) respectively due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (BLI verified) (Cat. No. FCM-M82W6) SDS-PAGE gel

Biotinylated Mouse FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Mouse FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (BLI verified) (Cat. No. FCM-M82W6) on SA Biosensor, can bind Herceptin with an affinity constant of 3.66 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Comments (0)

This web search service is supported by Google Inc.

totop