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Your Position: Home > Fc Receptor Proteins > FcRn (FCGRT & B2M) > Biotinylated Mouse FcRn/FCGRT & B2M Heterodimer Protein

Biotinylated Mouse FcRn/FCGRT & B2M, Avi Tag (Avitag™)

  • Synonym
    FcRn,FCGRT & B2M
  • Source
    MABSol® Biotinylated Mouse FcRn / FCGRT & B2M Heterodimer Protein (FCM-M82W6) is expressed from human HEK293 cells. It contains AA Ser 22 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q61559 (FCGRT) & P01887 (B2M)).
    Predicted N-terminus: Ser 22 (FCGRT) & Ile 21 (B2M)
  • Molecular Characterization
    FcRn(Ser 22 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M)) Q61559 (FCGRT) & P01887 (B2M)

    The subunit FCGRT carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The subunit Beta-2 microglobulin (B2M) carries a Twin Strep tag at the C-terminus. The protein has a calculated MW of 34.7 kDa (FCGRT), 14.7 kDa (B2M) . The protein migrates as 45-50 kDa (FCGRT), 15 kDa (B2M) respectively on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse FcRn / FCGRT & B2M (Cat. No. FCM-M82W6) SDS-PAGE gel

Biotinylated Mouse FcRn / FCGRT & B2M on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Biotinylated Mouse FcRn / FCGRT & B2MBiotinylated Mouse FcRn / FCGRT & B2M (Cat. No. FCM-M82W6) ELISA bioactivity

Immobilized Yervoy Ipilimumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse FcRn / FCGRT & B2M (Cat. No. FCM-M82W6) with a linear range of 0.01-0.3 μg/mL (QC tested).

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Price(USD) : $350.00

Price(USD) : $1380.00

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