Biotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 34.7 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag and subunit Beta-2 microglobulin (B2M) is fused with a Strep II tag at the C-terminus. The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 14 kDa (B2M) respectively due to different glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Biotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR verified) (Cat. No. FCM-R82W7) captured on Biotin CAP-Series S Sensor Chip can bind Herceptin® with an affinity constant of 10.3 nM as determined in a SPR assay (Biacore T200) (QC tested).
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