Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.1 kDa (FCGRT) and 13.0 kDa (B2M). Subunit FCGRT is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag and subunit Beta-2 microglobulin (B2M) is fused with a Strep II tag at the C-terminus. The reducing (R) protein migrates as 40 kDa (FCGRT) and 14 kDa (B2M) respectively due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Captured Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag (Cat. No. FCM-C82W5) on Biotin CAP - Series S sensor Chip can bind Herceptin® with an affinity constant of 0.241 μM as determined in a SPR assay (Biacore T200) (QC tested).
Loaded Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag (Cat. No. FCM-C82W5) on SA Biosensor, can bind Herceptin with an affinity constant of 0.075 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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