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Your Position: Home > Fc Receptor Proteins > FcRn (FCGRT & B2M) > FCM-C82W5

Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag™&Strep II Tag (BLI verified)

  • Synonym
    FcRn,FCGRT & B2M
  • Source
    MABSol® Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag (BLI verified) (FCM-C82W5) is expressed from human HEK293 cells. It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q8SPV9-1 (FCGRT) & Q8SPW0-1 (B2M)). In the region Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M), the AA sequence of Cynomolgus and Rhesus macaque FcRn (FCGRT & B2M) are homologus.
    Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)
  • Molecular Characterization
    Online(Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M)) Q8SPV9-1 (FCGRT) & Q8SPW0-1 (B2M)

    Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag (BLI verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 34.1 kDa (FCGRT) and 13.0 kDa (B2M). Subunit FCGRT is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag and subunit Beta-2 microglobulin (B2M) is fused with a Strep II tag at the C-terminus. The reducing (R) protein migrates as 40 kDa (FCGRT) and 14 kDa (B2M) respectively due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag (BLI verified) (Cat. No. FCM-C82W5) SDS-PAGE gel

Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag (BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Captured Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag (BLI verified) (Cat. No. FCM-C82W5) on Biotin CAP - Series S sensor Chip can bind Herceptin® with an affinity constant of 0.241 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag (BLI verified) (Cat. No. FCM-C82W5) on SA Biosensor, can bind Herceptin with an affinity constant of 0.075 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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