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Product Details
Synonyms
CD4, CD4mut, LEU3
Source
Human CD4 Protein, Fc Tag (CD4-H5259) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396 (Accession # AAH25782).
Predicted N-terminus: Lys 26
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 67.7 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.2 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris, Glycine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human CD4 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human CD4 Protein, Fc Tag (Cat. No. CD4-H5259) is more than 95% and the molecular weight of this protein is around 140-180 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human CD4 Protein, Fc Tag (Cat. No. CD4-H5259) at 1 μg/mL (100 μL/well) can bind Ibalizumab (Human IgG4) with a linear range of 0.2-3 ng/mL (QC tested).
Protocol
Immobilized HIV-1 [HIV-1/Clade B/C (CN54)] GP120, His Tag (Cat. No. GP4-V15227) at 2 μg/mL (100 μL/well) can bind Human CD4 Protein, Fc Tag (Cat. No. CD4-H5259) with a linear range of 1-31 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human CD4 Protein, Fc Tag (Cat. No. CD4-H5259) captured on Protein A Chip can bind Biotinylated HIV-1 [HIV-1/Clade B/C (CN54)] GP120, His,Avitag (Cat. No. GP0-V182E6) with an affinity constant of 188 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
T-cell surface antigen CD4
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
25 Details
- Highest Development Stage：
Approved
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