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Your Position: Home > Bio-Markers & CD Antigens > Glycophorin A > Mouse Glycophorin A / CD235a Protein, His Tag & Fc Tag

Mouse Glycophorin A / CD235a Protein, His Tag & Fc Tag

  • Synonym
    CD235a,Glycophorin-A,Gypa
  • Source
    Mouse Glycophorin A, His Tag & Fc Tag (CDA-M526x) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Val 108 (Accession # P14220).
    Predicted N-terminus: Met
  • Molecular Characterization
    CD235a(Met 1 - Val 108) P14220

    This protein carries a human IgG1 Fc tag at the N-terminus and a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 39.6 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse Glycophorin A, His Tag & Fc Tag (Cat. No. CDA-M526x) SDS-PAGE gel

Mouse Glycophorin A, His Tag & Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

  • Background
    Glycophorin-A, a member of the glycophorin-A family, is also known as CD235a and Gypa, which is heavily glycosylated on serine and threonine residues at the N-terminal extracellular domain. CD235a,a single-pass type III membrane protein,is the major intrinsic membrane sialoglycoprotein of the erythrocyte. Also,CD235a appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. Furthermore,CD235a may be involved in translocation of SLC4A1 to the plasma membrane.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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