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Your Position: Home > Bio-Markers & CD Antigens > Human-CD19-Protein-C-Fc-Tag

Human CD19 (20-291) Protein, Fc Tag

  • Synonym
  • Source
    Human CD19 (20-291), Fc Tag (CD9-H5259) is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291 (Accession # AAH06338).
    Predicted N-terminus: Pro 20
  • Molecular Characterization
    CD19(Pro 20 - Lys 291) AAH06338

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 56.3 kDa. The protein migrates as 56-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

Human CD19 (20-291), Fc Tag (Cat. No. CD9-H5259) SDS-PAGE gel

Human CD19 (20-291), Fc Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Human CD19 (20-291), Fc TagHuman CD19 (20-291), Fc Tag (Cat. No. CD9-H5259) ELISA bioactivity

Immobilized FMC63 MAb at 2 μg/mL (100 μL/well) can bind Human CD19, Fc Tag (Cat. No. CD9-H5259) with a linear range of 0.02-0.313 μg/mL (QC tested).

Evaluation of CAR expression

FACS Analysis of  anti-CD19 CAR expression

293 cells were transfected with FCM63-scFv and RFP tag .  2x105 of the cells were first incubated with A. Human Fc tag control. B. Recombinant  human CD19,Fc Tag (Cat. No. CD9-H5259, 10ug/ml ). C. Recombinant  human CD19,Fc Tag(Cat. No. CD9-H5259, 10ug/ml ) and FMC63(Mouse anti-CD19 antibody) . The FITC- anti-human IgG Fc was used to analyse with FACS. RFP was used to evaluate CAR(FMC63-scFv) expression and FITC was used to evaluate the binding activity  of recombinant  human CD19,Fc Tag .

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