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Your Position: Home > Other Recombinant Proteins > Integrin alpha V beta 6 > Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein

Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein

  • Synonym
    Integrin alpha V beta 6,ITGAV&ITGB6
  • Source
    Human ITGAV & ITGB6 Heterodimer Protein (IT6-H52E1) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gly 22 - Asn 707 (ITGB6) (Accession # NP_002201 (ITGAV)& AAI21179 (ITGB6)).
    Predicted N-terminus: Phe 31 (ITGAV) & Gly 22 (ITGB6)
  • Molecular Characterization
    ITGAV&ITGB6(Phe 31 - Val 992 (ITGAV) & Gly 22 - Asn 707 (ITGB6)) NP_002201 (ITGAV)& AAI21179 (ITGB6)

    Human ITGAV & ITGB6 Heterodimer Protein, produced by co-expression of ITGAV and ITGB6, has a calculated MW of 113 kDa (ITGAV) and 79.6 kDa (ITGB6). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB6 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 31 (ITGAV) & Gly 22 (ITGB6). The reducing (R) protein migrates as 145 kDa (ITGAV) and 110-130 kDa (ITGB6) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, 2 mM CaCl2. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ITGAV & ITGB6 Heterodimer Protein (Cat. No. IT6-H52E1) SDS-PAGE gel

Human ITGAV & ITGB6 Heterodimer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human ITGAV & ITGB6 Heterodimer ProteinHuman ITGAV & ITGB6 Heterodimer Protein (Cat. No. IT6-H52E1) ELISA bioactivity

Immobilized Human ITGAVB6, His Tag (Cat. No. IT6-H52E1) at 2μg/mL (100 μL/well) can bind Human TGF-Beta 1 / TGFB1, His Tag & Avi Tag with a linear range of 2-39 ng/mL (QC tested).

Human ITGAV & ITGB6 Heterodimer ProteinHuman ITGAV & ITGB6 Heterodimer Protein (Cat. No. IT6-H52E1) ELISA bioactivity

Immobilized Human Fibronectin at 10μg/mL (100μL/well) can bind Human ITGAV&ITGB6 (Cat. No. IT6-H52E1) with a linear range of 0.313-2.5ug/mL (Routinely tested).

Human ITGAV & ITGB6 Heterodimer ProteinHuman ITGAV & ITGB6 Heterodimer Protein (Cat. No. IT6-H52E1) ELISA bioactivity

Immobilized Human Vitronectin at 5μg/mL (100μL/well) can bind Human ITGAV&ITGB6 (Cat. No. IT6-H52E1) with a linear range of 0.625-20ug/mL (Routinely tested).

  • Background
    Integrin alpha V beta 6 is a heterodimer of beta-6 associating with alpha-V. Integrin alpha-V beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. Also, Integrin alpha-V beta-6 acts as a receptor for coxsackievirus A9 and coxsackievirus B1 as well as herpes simplex virus-1/HHV-1. Furthermore, it binds the TGF-beta latency‑associated peptide (LAP) and activates TGF-beta 1 or TGF-beta 3 from large latent complexes. This activation requires interaction with LTBP-1 and fibronectin, and is enhanced by PAR-1.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $410.00

Price(USD) : $2880.00

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