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Your Position: Home > MABSol® Biotin Labeled Proteins > CD47 > Biotinylated Human CD47 Protein, Fc Tag

Biotinylated Human CD47, Fc Tag, Avi Tag (Avitag™)

  • Synonym
  • Source
    MABSol® Biotinylated Human CD47, Fc Tag (CD7-H82F6) is expressed from human HEK293 cells. It contains AA Gln 19 - Pro 139 (Accession # NP_942088).
    Predicted N-terminus: Gln 19
  • Molecular Characterization
    CD47(Gln 19 - Pro 139) NP_942088

    This protein carries a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 42.2 kDa. The protein migrates as 50 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human CD47, Fc Tag (Cat. No. CD7-H82F6) SDS-PAGE gel

Biotinylated Human CD47, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Biotinylated Human CD47, Fc TagBiotinylated Human CD47, Fc Tag (Cat. No. CD7-H82F6) ELISA bioactivity

Immobilized Human SIRP alpha, His Tag, Strep Tag (Cat. No. SIA-H5225) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD47, Fc Tag (Cat. No. CD7-H82F6) with a linear range of 0.008-0.25 μg/mL (QC tested).

  • Background
    Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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