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Your Position: Home > Immune Checkpoint Proteins > CD47 > Biotinylated Human CD47 Protein, Fc,Avitag

Biotinylated Human CD47 Protein, Fc,Avitag™

  • Synonym
    CD47,MER6,IAP,OA3
  • Source
    MABSol® Biotinylated Human CD47, Fc,Avitag (CD7-H82F6) is expressed from human HEK293 cells. It contains AA Gln 19 - Pro 139 (Accession # NP_942088).
    Predicted N-terminus: Gln 19
  • Molecular Characterization
    Online(Gln 19 - Pro 139) NP_942088

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 42.2 kDa. The protein migrates as 50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) SDS-PAGE gel

Biotinylated Human CD47, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human CD47, Fc,AvitagBiotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) ELISA bioactivity

Immobilized Human SIRP alpha, His Tag (HPLC-verified) (Cat. No. SIA-H5225) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) with a linear range of 0.008-0.25 μg/mL (QC tested).

Biotinylated Human CD47, Fc,AvitagBiotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) ELISA bioactivity

Immobilized Biotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Cynomolgus SIRP alpha, His Tag (Cat. No. SIA-C52H7) with a linear range of 0.039-0.625 μg/mL (Routinely tested).

Biotinylated Human CD47, Fc,AvitagBiotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) ELISA bioactivity

Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human SIRP alpha, Mouse IgG1 Fc Tag (HPLC-verified) (Cat. No. SIA-H52A8): Biotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 0.2006 μg/mL (Routinely tested).

Biotinylated Human CD47, Fc,AvitagBiotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) ELISA bioactivity

Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human SIRP alpha, His Tag (HPLC-verified) (Cat. No. SIA-H5225): Biotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 0.1245 μg/mL (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

FACS assay shows that Biotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) can bind to ACHN cell expressing human SIRP-a. The concentration of CD47 used is 3 μg/mL (Routinely tested).

FACS assay shows that recombinant Biotinylated FACS

FACS analysis shows that the binding of Human CD47 to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of Human CD47 used is 3 μg/mL.

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Price(USD) : $350.00

Price(USD) : $1380.00

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