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Your Position: Home > Bio-Markers & CD Antigens > Human MS4A1 / CD20 Protein, TrxA Tag

Human MS4A1 / CD20 Protein, TrxA Tag

  • Synonym
    MS4A1,CD20,MS4A-1
  • Source
    Human CD20 Protein, TrxA Tag (CD0-H5143) is expressed from E.coli cells. It contains AA Ile 141 - Ser 188 (Accession # NP_068769).
    Predicted N-terminus: Met
  • Molecular Characterization
    MS4A1(Ile 141 - Ser 188) NP_068769

    This protein carries a trxA at the N-terminus, and has a calculated MW of 22.7 kDa. The predicted N-terminus is Met. The reducing (R) protein migrates as 25 kDa in SDS-PAGE.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD20 Protein, TrxA Tag (Cat. No. CD0-H5143) SDS-PAGE gel

Human CD20 Protein, TrxA Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Human CD20 Protein, TrxA TagHuman CD20 Protein, TrxA Tag (Cat. No. CD0-H5143) ELISA bioactivity

Immobilized Anti-CD20 at 5 μg/mL (100 μL/well) can bind Human MS4A1, TrxA Tag (Cat. No. CD0-H5143) with a linear range of 0.039-0.313 μg/mL (QC tested).

  • Background
    B-lymphocyte antigen CD20 is also known as B-lymphocyte surface antigen B1, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1 and MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. CD20 is expressed on all stages of B cell development except the first and last; it is present from late pro-B cells through memory cells, but not on either early pro-B cells or plasma blasts and plasma cells. It is found on B-cell lymphomas, hairy cell leukemia, B-cell chronic lymphocytic leukemia, and melanoma cancer stem cells. The protein has no known natural ligand and its function is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that it acts as a calcium channel in the cell membrane. CD20 / MS4A1 is the target of the monoclonal antibodies (mAb) rituximab, Ibritumomab tiuxetan, and tositumomab, which are all active agents in the treatment of all B cell lymphomas and leukemias. Defects in CD20 / MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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