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Your Position: Home > Cytokines & Growth Factors > Biotinylated Human PDGF-BB Protein, His Tag, Avi Tag

Human PDGF-BB Protein, His Tag, Avi Tag (Avitag™)

  • Synonym
    PDGFBB,PDGFB,FLJ12858,PDGF2,SIS,SSV,c-sis
  • Source
    Human PDGF-BB Protein, His Tag, Avi Tag (PDB-H5127) is expressed from E.coli cells. It contains AA Ser 82 - Thr 190 (Accession # AAH29822).
    Predicted N-terminus: Ser 82
  • Molecular Characterization
    PDGF-B(Ser 82 - Thr 190) AAH29822

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 15 kDa. the protein migrates as 17 kDa under reducing (R) condition, and 35 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 0.085% TFA in 30% ACN. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human PDGF-BB Protein, His Tag, Avi Tag (Cat. No. PDB-H5127) SDS-PAGE gel

Human PDGF-BB Protein, His Tag, Avi Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human PDGF-BB Protein, His Tag, Avi TagHuman PDGF-BB Protein, His Tag, Avi Tag (Cat. No. PDB-H5127) ELISA bioactivity

Immobilized Human PDGF-BB, His Tag, Avi Tag (Cat. No. PDB-H5127) at 5 µg/mL (100 µl/well) can bind Human PDGF R beta, Fc Tag (Cat. No. PDB-H5252) with a linear range of 0.02-1.2 µg/mL (QC tested).

  • Background
    PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in inductive events involved in patterning and morphogenesis. In addition to driving mesenchymal proliferation, PDGFs have been shown to direct the migration, differentiation and function of a variety of specialised mesenchymal and migratory cell types, both during development and in the adult animal. Other growth factors in this family include vascular endothelial growth factors B and C (VEGF-B, VEGF-C)which are active in angiogenesis and endothelial cell growth, and placenta growth factor (PlGF) which is also active in angiogenesis. PDGF plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis. PDGF is a required element in cellular division for fibroblast, a type of connective tissue cell. PDGF is also known to maintain proliferation of oligodendrocyte progenitor cells. Platelet-derived growth factor subunit B is also known as PDGFB, FLJ12858, PDGF2, SIS, SSV, c-sis, is a member of the platelet-derived growth factor family. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $300.00

Inventory : In stock

Price(USD) : $550.00

Inventory : In stock

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