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Biotinylated Human TNF-alpha (HPLC-verified), Avi Tag (Avitag™)

  • Synonym
  • Source
    MABSol® Biotinylated Human TNF-alpha (TNA-H82E3) is expressed from human HEK293 cells. It contains AA Val 77 - Leu 233 (Accession # NP_000585.2).
    Predicted N-terminus: Val 77
  • Molecular Characterization
    TNF-alpha(Val 77 - Leu 233) NP_000585.2

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) The protein has a calculated MW of 19.2 kDa. The protein migrates as 21 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation. More than 95% of Biotinylated Human TNF-alpha (TNA-H82E3) presents as active trimers with a molecular mass of 57 kDa, as determined by SEC-HPLC analysis.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human TNF-alpha (HPLC-verified) (Cat. No. TNA-H82E3) SDS-PAGE gel

Biotinylated Human TNF-alpha (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Biotinylated Human TNF-alpha (HPLC-verified) (Cat. No. ) HPLC images

Over 95% of Biotinylated Human TNF-alpha (TNA-H82E3) presents as active trimers with a molecular mass of 57 kDa, as determined by SEC-HPLC analysis.

Biotinylated Human TNF-alpha (HPLC-verified)Biotinylated Human TNF-alpha (HPLC-verified) (Cat. No. TNA-H82E3) ELISA bioactivity

Immobilized Humira at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, His Tag (Cat. No. TNA-H82E3) with a linear range of 0.4-6 ng/mL (QC tested).

Human FcRn Heterodimer Protein Cell_Base

Recombinant Human TNF-alpha (Cat.No. TNA-H82E3) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is 0.029-0.052ng/ml (Routinely tested).

Human FcRn Heterodimer Protein Cell_Base

Neutralization assay shows that the cytotoxicity effect of TNF-alpha (Cat.No. TNA-H82E3) was inhibited by increasing concentration of Adalimumab. The concentration of TNF-alpha used is 1ng/mL. The IC50 is 7ng/mL (Routinely tested).

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