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Your Position: Home > Cytokines > TNF-alpha > Biotinylated Mouse TNF-alpha Protein, His Tag (HPLC-verified)

Biotinylated Mouse TNF-alpha (HPLC-verified), Avi Tag (Avitag™)

  • Synonym
    DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
  • Source
    MABSol® Biotinylated Mouse TNF-alpha, His Tag, Avi Tag (HPLC-verified) (TNA-M82E9) is expressed from human HEK293 cells. It contains AA Leu 80 - Leu 235 (Accession # P06804).
    Predicted N-terminus: Leu 80
  • Molecular Characterization
    Online(Leu 80 - Leu 235) P06804

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 20.2 kDa. The protein migrates as 21 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.8-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse TNF-alpha, His Tag, Avi Tag (HPLC-verified) (Cat. No. TNA-M82E9) SDS-PAGE gel

Biotinylated Mouse TNF-alpha, His Tag, Avi Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
Biotinylated Mouse TNF-alpha, His Tag, Avi Tag (HPLC-verified) (Cat. No. ) HPLC images

The purity of Biotinylated Mouse TNF-alpha, His Tag, Avi Tag (HPLC-verified) (Cat. No. TNA-M82E9) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA
Biotinylated Mouse TNF-alpha, His Tag, Avi Tag (HPLC-verified)Biotinylated Mouse TNF-alpha, His Tag, Avi Tag (HPLC-verified) (Cat. No. TNA-M82E9) ELISA bioactivity

Immobilized Human TNFR1, Fc Tag (Cat. No. TN1-H5251) at 5 µg/mL (100 µl/well) can bind Biotinylated Mouse TNF-alpha, His Tag, Avi Tag (HPLC-verified) (Cat. No. TNA-M82E9) with a linear range of 0.8-12.5 ng/mL (QC tested).

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Price(USD) : $1380.00

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