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Your Position: Home > Cytokines > TNF-alpha > Biotinylated Mouse TNF-alpha Protein, His Tag

Biotinylated Mouse TNF-alpha (HPLC-verified), Avi Tag (Avitag™)

  • Synonym
    DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
  • Source
    MABSol® Biotinylated Mouse TNF-alpha Protein, His Tag (TNA-M82E9) is expressed from human HEK293 cells. It contains AA Leu 80 - Leu 235 (Accession # P06804).
    Predicted N-terminus: Leu 80
  • Molecular Characterization
    TNF-alpha(Leu 80 - Leu 235) P06804

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) The protein has a calculated MW of 20.2 kDa. The protein migrates as 21 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation. More than 90% of Biotinylated Mouse TNF-alpha Protein, His Tag (TNA-M82E9) presents as active trimers with a molecular mass of 60 kDa, as determined by SEC-HPLC analysis.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.8-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse TNF-alpha Protein, His Tag (Cat. No. TNA-M82E9) SDS-PAGE gel

Biotinylated Mouse TNF-alpha Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
Biotinylated Mouse TNF-alpha Protein, His Tag (Cat. No. ) HPLC images

Over 90% of Biotinylated Mouse TNF-alpha Protein, His Tag (TNA-M82E9) presents as active trimers with a molecular mass of 60 kDa, as determined by SEC-HPLC analysis.

Bioactivity
Biotinylated Mouse TNF-alpha Protein, His TagBiotinylated Mouse TNF-alpha Protein, His Tag (Cat. No. TNA-M82E9) ELISA bioactivity

Immobilized Human TNFR1, Fc Tag (Cat. No. TN1-H5251) at 5 µg/mL (100 µl/well) can bind Biotinylated Mouse TNF-alpha Protein, His Tag (Cat. No. TNA-M82E9) with a linear range of 0.8-12.5 ng/mL (QC tested).

  • Background
    Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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