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Your Position: Home > Isotype Controls > IgG2a Fc > Biotinylated Mouse IgG2a Fc Protein, Avitag

Biotinylated Mouse IgG2a Fc Protein, Avitag™

  • Synonym
    IgG2A
  • Source
    Biotinylated Mouse IgG2a Fc, Avitag (IGA-M8210) is expressed from human 293 cells (HEK293). It contains AA Glu 98 - Lys 330 (Accession # P01863).
    Predicted N-terminus: Glu 98
  • Molecular Characterization
    Online(Glu 98 - Lys 330) P01863

    This protein carries an Avi tag at the C-terminus.

    The protein has a calculated MW of 28.2 kDa. The protein migrates as 36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.7-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse IgG2a Fc, Avitag (Cat. No. IGA-M8210) SDS-PAGE gel

Biotinylated Mouse IgG2a Fc, Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Mouse IgG2a Fc, AvitagBiotinylated Mouse IgG2a Fc, Avitag (Cat. No. IGA-M8210) ELISA bioactivity

Immobilized Recombinant Protein A at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IgG2a Fc, His Tag, Avi Tag (Cat. No. IGA-M8210) with a linear range of 0.4-12.5 ng/mL (QC tested).

Biotinylated Mouse IgG2a Fc, AvitagBiotinylated Mouse IgG2a Fc, Avitag (Cat. No. IGA-M8210) ELISA bioactivity

Immobilized Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IgG2a Fc, His Tag, Avi Tag (Cat. No. IGA-M8210) with a linear range of 0.01-0.313 μg/mL (Routinely tested).

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Price(USD) : $350.00

Price(USD) : $1650.00

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