This is the new website for acrobiosystems.com. We would love to hear what you think of it - please send feedback

Welcome to ACROBiosystems

0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Bio-Markers & CD Antigens > Cynomolgus PCSK9 Protein

Cynomolgus PCSK9 Protein

  • Synonym
    PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
  • Source
    Cynomolgus PCSK9, His Tag (PC9-C5223) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # G7NVZ1).
    Predicted N-terminus: Gln 31
  • Molecular Characterization
    PCSK9(Gln 31 - Gln 692) G7NVZ1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 73.2 kDa. The protein migrates as 20 kDa and 65 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus PCSK9, His Tag (Cat. No. PC9-C5223) SDS-PAGE gel

Cynomolgus PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Cynomolgus PCSK9, His TagCynomolgus PCSK9, His Tag (Cat. No. PC9-C5223) ELISA bioactivity

Immobilized Human LDL R, Strep Tag (Cat. No. LDR-H5281) at 2 μg/mL (100 μL/well) can bind Cynomolgus PCSK9, His Tag (Cat. No. PC9-C5223) with a linear range of 4-31 ng/mL (QC tested).

  • Background
    Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)

Order Details

Price(USD) : $260.00

Inventory : In stock

Price(USD) : $1980.00

Inventory : In stock

Datasheet & Documentation

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Related Products

Questions & Comments

This web search service is supported by Google Inc.