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Your Position: Home > FabAffinity Chromatography Media > FabAffinity KBP Prepacked Column 1ml

FabAffinity KBP Prepacked Column 1ml

FabAffinity KBP Agarose High Flow Resin, with KBP (human Kappa chain binding protein) coupled to high flow Agarose matrix, is a new choice for purification of human antibodies and antibody fragments containing the constant domain of the Kappa light chain.This in-house developed proprietary ligand, derived from HEK293 cell, can bind to the constant domain of the kappa light chain (i.e. fragments lacking the constant region of the light chain will not bind), therefore capable of binding target molecules containing the constant region of the light chain, for example, Fab, IgG, IgA and IgM.
Features
  1. High kappa chain binding affinity validated by SPR with Kd in the nmol range

  2. Efficient capture of human antibodies and antibody fragments containing the constant region of the Kappa light chain, even from complex sources at very low concentrations.

  3. Good chemical stability and easy cleaning with long life time

  4. Single point attachment coupling chemistry gives better ligand accessibility for higher binding capacity

Specification
CompositionHighly cross-linked Agarose
Average particle size90 micron
Form1ml/5ml prepacked column;
LigandrKBP derived from HEK293
Coupling chemistryEpoxy
Dynamic binding capacity110mg Fab /1ml prepacked column;
50mg Fab /5ml prepacked column
Recommended flow rate0.2-1 ml/min for 1ml prepacked column;
1-5ml/min for 5ml prepacked column
Column dimension0.7x2.5 cm for 1ml prepacked column;
1.6x2.5 cm for 5ml prepacked column
Maximum Pressure Drop0.3Mpa
Chemical stabilityStable in all aqueous buffers commonly used in KBP affinity chromatography
pH working range3-10
pH CIP range (short term)2-11
CIP stability6M Urea, 5mM NaOH, 0.1M acetic acid, 30% IPA
Temperature stability12-40oC
Storage20% Ethanol
Shelf life5 years
  1. Delivered at room temperature, and recommended long-term storage at 2-8 °C

FabAffinity KBP Agarose High Flow Resin belongs to the MabAffinity resins family for capture of monoclonal antibodies (MAbs), and is available with different pack sizes for easy Mabs purification, process development and scale up. Immunoprecipitation studies with FabAffinity KBP Agarose High Flow are also commonly used to purify proteins or protein complexes.

For more information and technical questions, Please contact us via TechSupport@acrobiosystems.com.

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Price(USD) : $220.00

Inventory : In stock

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