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Your Position: Home > All Other Proteins > Integrin alpha V beta 3 > Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein

Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein

  • Synonym
    Integrin alpha V beta 3,ITGAV&ITGB3
  • Source
    Human ITGAV & ITGB3 Heterodimer Protein (IT3-H52E3) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gly 27 - Asp 718 (ITGB3) (Accession # NP_002201 (ITGAV) & AAI27667.1 (ITGB3)).
    Predicted N-terminus: Phe 31 (ITGAV) & Gly 27 (ITGB3)
  • Molecular Characterization
    Online(Phe 31 - Val 992 (ITGAV) & Gly 27 - Asp 718 (ITGB3)) NP_002201 (ITGAV) & AAI27667.1 (ITGB3)

    Human ITGAV & ITGB3 Heterodimer Protein, produced by co-expression of ITGAV and ITGB3, has a calculated MW of 113.0 kDa (ITGAV) and 81.8 kDa (ITGB3). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB3 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 115-135 kDa and 140-150 kDa (ITGAV) and 85-100 kDa (ITGB3) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, 2 mM CaCl2. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ITGAV & ITGB3 Heterodimer Protein (Cat. No. IT3-H52E3) SDS-PAGE gel

Human ITGAV & ITGB3 Heterodimer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human ITGAV & ITGB3 Heterodimer ProteinHuman ITGAV & ITGB3 Heterodimer Protein (Cat. No. IT3-H52E3) ELISA bioactivity

Immobilized Human Fibronectin at 2 μg/mL (100 μL/well) can bind Human ITGAV & ITGB3 Heterodimer Protein (Cat. No. IT3-H52E3) with a linear range of 0.01-0.313 μg/mL (QC tested).

Human ITGAV & ITGB3 Heterodimer ProteinHuman ITGAV & ITGB3 Heterodimer Protein (Cat. No. IT3-H52E3) ELISA bioactivity

Immobilized Human Vitronectin at 2 μg/mL (100 μL/well) can bind Human ITGAV & ITGB3 Heterodimer Protein (Cat. No. IT3-H52E3) with a linear range of 10-156 ng/mL (Routinely tested).

  • Background
    Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Also, Integrin alpha-V/beta-3 acts as a receptor for herpes virus 8/HHV-8, coxsackievirus A9, Hantaan virus, cytomegalovirus/HHV-5, human metapneumovirus, human parechovirus 1 and west nile virus. Furthermore, in case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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