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Your Position: Home > MABSol® Biotin Labeled Proteins > PD-L2 > Biotinylated Human PD-L2 / B7-DC Protein (recommended for biopanning), Avi Tag (Avitag™)

Biotinylated Human PD-L2 / B7-DC Protein (recommended for biopanning), Avi Tag (Avitag™)

  • Synonym
    PDL2,PD-L2,Butyrophilin B7-DC,CD273,PDCD1 ligand 2,PDCD1L2,PDCD1LG2
  • Source
    Biotinylated Human PD-L2(recommended for biopanning) (PD2-H82E8) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 219 (Accession # AAI13679).
    Predicted N-terminus: Leu 20
  • Molecular Characterization
    PD-L2(Leu 20 - Pro 219) AAI13679

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 25.2 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Application
    PD2-H82E8 works best for experiments that test the binding between PD-L2 and candidate antibodies, such as biopanning and other relevant assays.
    This product is NOT suitable for testing PD1-PDL2 binding. For this type of application, we strongly recommend you to choose PD2-H82F6 as an alternative.
  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human PD-L2(recommended for biopanning) (Cat. No. PD2-H82E8) SDS-PAGE gel

Biotinylated Human PD-L2(recommended for biopanning) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Biotinylated Human PD-L2(recommended for biopanning)Biotinylated Human PD-L2(recommended for biopanning) (Cat. No. PD2-H82E8) ELISA bioactivity

Immobilized anti-PD-L2 mAb (human IgG1) at 2 μg/mL (100 μl/well) can bind Biotinylated Human PD-L2, Avi Tag (Avitag™) (Cat. No. PD2-H82E8) with a linear range of 3-50 ng/mL (QC tested).

  • Citations
  • Background
    Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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