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This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 39.6 kDa. The protein migrates as 55-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Nectin-3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human Nectin-3, His Tag (Cat. No. PV3-H52E4) is more than 85% and the molecular weight of this protein is around 65-90 kDa verified by SEC-MALS.
Immobilized Human Nectin-3, His Tag (Cat. No. PV3-H52E4) at 2 μg/mL (100 μL/well) can bind Human PVRL1, Fc Tag with a linear range of 1-20 ng/mL (QC tested).
Loaded Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) on Protein A Biosensor, can bind Human Nectin-3, His Tag (Cat. No. PV3-H52E4) with an affinity constant of 2.7 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human Nectin-3, His Tag (Cat. No. PV3-H52E4) on HIS1K Biosensor, can bind Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) with an affinity constant of 4.99 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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